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This page is an AI-assisted Turkish summary of the study presented at the 26th World Congress of Psychiatry. The study belongs to the presenting authors; please refer to the original source when citing it.

EV0945

Modulation of mitochondrial respiration and hydrogen peroxide production by antioxidants and phosphorylated tau

MODULATION OF MITOCHONDRIAL RESPIRATION AND HYDROGEN PEROXIDE PRODUCTION BY ANTIOXIDANTS AND PHOSPHORYLATED TAU

Curcumin increases complex I activity in isolated mitochondria while reducing complex II+III activity and reactive oxygen species production in a dose-dependent manner.

The aim of the study was to investigate the effects of curcumin, purpurin, epicatechin, and methylene blue, which have attracted interest as potential antioxidant treatments for Alzheimer’s disease, on mitochondrial respiration, electron transport chain (ETC) complex I and complex II+III activity, and reactive oxygen species (ROS) production. Isolated pig brain mitochondria were used as an in vitro biological model; hydrogen peroxide formation was measured by fluorescence using the Amplex Red probe, while ETC complex activities were determined spectrophotometrically. The drugs were tested at a concentration of 10 µmol/L, and phosphorylated tau (P-tau) was evaluated over a range of 3 to 120 nmol/L; values were reported as the mean ± SD of at least three independent measurements, and statistical significance was assessed using a one-sample t-test with the control value set at 100%. The most pronounced effects were observed with purpurin and curcumin; both significantly reduced complex II+III activity (p < 0.01). Curcumin significantly increased complex I activity (p < 0.05); epicatechin and purpurin had no effect on complex I. Curcumin produced the most pronounced and dose-dependent effect on ROS production (measured at concentrations of 1, 5, 20, and 500 µmol/L), whereas no significant effect of P-tau was detected. According to the authors, curcumin may suppress succinate-dependent pathways by selectively increasing NADH oxidation through complex I, and it significantly reduces ROS production; therefore, further testing of curcumin as a drug for Alzheimer’s disease, in conjunction with P-tau oligomers, is planned.

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